Coding

Part:BBa_K4291002

Designed by: Zhang Jiashu   Group: iGEM22_Canton_HS   (2022-08-11)


tnaC

tnaC

The tnaC regulatory gene from the tna operon of E. coli controls the transcription of its own operon through an attenuation mechanism relying on the accumulation of arrested ribosomes during inhibition of its own translation termination. In this project, the expression of tnaC was regulated by tnaCAB operon in the pTrc99K vector. This mechanism requires ribosomal arrest induced by the regulatory nascent TnaC peptide in response to free L-tryptophan (L-Trp).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None